Inherently Electroactive Graphene Oxide Nanoplatelets As Labels for Single Nucleotide Polymorphism Detection
- Alessandra Bonanni, Chun Kiang Chua, Guanjia Zhao, Zdeněk Sofer, Martin Pumera*
Graphene materials are being widely used in electrochemistry due to their versatility and excellent properties as platforms for biosensing. However, no records show the use of inherent redox properties of graphene oxide as a label for detection. Here for the first time we used graphene oxide nanoplatelets (GONPs) as electroactive labels for DNA analysis. The working signal comes from the reduction of he oxygen-containing groups present on the surface of GONPs. The different ability of the graphene oxide nanoplatelets to conjugate to DNA hybrids obtained with complementary, noncomplementary, and one-mismatch sequences allows the discrimination of single-nucleotide polymorphism correlated with Alzheimer's disease. We believe that our findings are very important to open a new route in the use of graphene oxide in electrochemistry.
Three-dimensionally printed electrochemical systems for biomedical analytical applications
Emerging mono-elemental 2D nanomaterials for electrochemical sensing applications: From borophene to bismuthene